Flow Cytometry analysis of U20S cells using anti-Cyclin T1 antibody (PB9990).
Overlay histogram showing U20S cells stained with PB9990 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin T1 Antibody (PB9990,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U937 cells using anti-Cyclin T1 antibody (PB9990).
Overlay histogram showing U937 cells stained with PB9990 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin T1 Antibody (PB9990,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

IF analysis of Cyclin T1 using anti-Cyclin T1 antibody (PB9990).
Cyclin T1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Cyclin T1 Antibody (PB9990) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.